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Image Search Results


A20 enhances BEECs autophagy. ( A ) Western Blot detection of changes in key autophagy proteins P62 and LC3II expression. ( B ) Quantitative analysis of P62, LC3II, and A20. Data are presented as the mean ± SEMs of three independent experiments. * p < 0.05, ** p < 0.01.

Journal: Animals : an Open Access Journal from MDPI

Article Title: A20 Attenuates Inflammatory Injury in Bovine Endometrial Epithelial Cells Through Autophagy-Mediated NLRP3 Inflammasome Inactivation

doi: 10.3390/ani15243513

Figure Lengend Snippet: A20 enhances BEECs autophagy. ( A ) Western Blot detection of changes in key autophagy proteins P62 and LC3II expression. ( B ) Quantitative analysis of P62, LC3II, and A20. Data are presented as the mean ± SEMs of three independent experiments. * p < 0.05, ** p < 0.01.

Article Snippet: Antibodies against A20 (Cat# sc-166692, RRID: AB_2204516) and NLRP3 (Cat# sc-518122) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Western Blot, Expressing

A20 attenuated LPS-induced inflammatory damage in BEECs. ( A , D ) The analysis of active Caspase-1 using flow cytometry. ( B , E ) The mRNA expression levels of IL-1β measured by qRT-PCR. ( C , F ) The release of LDH detected in the supernatant. The data were presented as the mean ± SEMs of three independent experiments. ** p < 0.01 vs. the NC group, and # p < 0.05, ## p < 0.01 vs. the NC + LPS group.

Journal: Animals : an Open Access Journal from MDPI

Article Title: A20 Attenuates Inflammatory Injury in Bovine Endometrial Epithelial Cells Through Autophagy-Mediated NLRP3 Inflammasome Inactivation

doi: 10.3390/ani15243513

Figure Lengend Snippet: A20 attenuated LPS-induced inflammatory damage in BEECs. ( A , D ) The analysis of active Caspase-1 using flow cytometry. ( B , E ) The mRNA expression levels of IL-1β measured by qRT-PCR. ( C , F ) The release of LDH detected in the supernatant. The data were presented as the mean ± SEMs of three independent experiments. ** p < 0.01 vs. the NC group, and # p < 0.05, ## p < 0.01 vs. the NC + LPS group.

Article Snippet: Antibodies against A20 (Cat# sc-166692, RRID: AB_2204516) and NLRP3 (Cat# sc-518122) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Flow Cytometry, Expressing, Quantitative RT-PCR

Inhibiting autophagy in BEECs weakened the effect of A20 on suppressing the activation of the NLRP3 inflammasome. ( A ) The protein expression levels of NLRP3, Pro-Caspase-1, Cleaved-Caspase-1, Pro-IL-1β, Cleaved-IL-1β, and ATG5 measured by Western blot. ( B ) Quantitative analysis of NLRP3, Pro-Caspase-1, Cleaved-Caspase-1, Pro-IL-1β, Cleaved-IL-1β and ATG5. The data were presented as the mean ± SEMs of three independent experiments. * p < 0.05, ** p < 0.01.

Journal: Animals : an Open Access Journal from MDPI

Article Title: A20 Attenuates Inflammatory Injury in Bovine Endometrial Epithelial Cells Through Autophagy-Mediated NLRP3 Inflammasome Inactivation

doi: 10.3390/ani15243513

Figure Lengend Snippet: Inhibiting autophagy in BEECs weakened the effect of A20 on suppressing the activation of the NLRP3 inflammasome. ( A ) The protein expression levels of NLRP3, Pro-Caspase-1, Cleaved-Caspase-1, Pro-IL-1β, Cleaved-IL-1β, and ATG5 measured by Western blot. ( B ) Quantitative analysis of NLRP3, Pro-Caspase-1, Cleaved-Caspase-1, Pro-IL-1β, Cleaved-IL-1β and ATG5. The data were presented as the mean ± SEMs of three independent experiments. * p < 0.05, ** p < 0.01.

Article Snippet: Antibodies against A20 (Cat# sc-166692, RRID: AB_2204516) and NLRP3 (Cat# sc-518122) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Activation Assay, Expressing, Western Blot

Schematic of A20 attenuating inflammatory damage in BEECs through autophagy-mediated inhibition of the NLRP3 inflammasome.

Journal: Animals : an Open Access Journal from MDPI

Article Title: A20 Attenuates Inflammatory Injury in Bovine Endometrial Epithelial Cells Through Autophagy-Mediated NLRP3 Inflammasome Inactivation

doi: 10.3390/ani15243513

Figure Lengend Snippet: Schematic of A20 attenuating inflammatory damage in BEECs through autophagy-mediated inhibition of the NLRP3 inflammasome.

Article Snippet: Antibodies against A20 (Cat# sc-166692, RRID: AB_2204516) and NLRP3 (Cat# sc-518122) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Inhibition